Experiment PCR to get started

[ctelmer]

ExperimentPCR[
{
Object[Sample, "id:L8kPEjn3rr5l"],
Object[Sample, "id:L8kPEjn3rr5l"]
},
{
{{Object[Sample, "id:aXRlGnR9O71O"], Object[Sample, "id:o1k9jAkVwNlr"]}},
{{Object[Sample, "id:aXRlGnR9O71O"], Object[Sample, "id:o1k9jAkVwNlr"]}}
},
ForwardPrimerVolume -> {
2.5 Microliter
},
ReversePrimerVolume -> {
2.5 Microliter
},
BufferVolume -> {
18 Microliter
},
ReactionVolume -> 50 Microliter,
MasterMix -> Model[Sample, "DreamTaq PCR Master Mix"],
MasterMixVolume -> 25 Microliter,
Activation -> True,
DenaturationTime -> 10 Second,
PrimerAnnealing -> True,
PrimerAnnealingTime -> 10 Second,
ExtensionTime -> 50 Second,
NumberOfCycles -> 25,
FinalExtension -> True,
HoldTemperature -> 4 Celsius
]

[ctelmer]

Then you can run the SamplesOut on a gel.

ExperimentAgaroseGelElectrophoresis[
{
Object[Sample,"id:E8zoYvzNJEBN"],
Object[Sample,"id:Y0lXejlMxaJl"]
},
Scale -> Analytical,
SampleVolume -> 5 Microliter,
LoadingDyeVolume -> 3 Microliter,
SampleLoadingVolume -> 8 Microliter
]

[ctelmer]

Or measure Absorbance at 260nm.

ExperimentAbsorbanceIntensity[
{
Object[Sample,"id:E8zoYvzNJEBN"],
Object[Sample,"id:Y0lXejlMxaJl"]
},
Wavelength -> 260 Nanometer,
ConsolidateAliquots -> False,
MeasureWeight -> False,
MeasureVolume -> False,
ImageSample -> False
]