This is an automated pipeline for the subcellular segmentation of 7 different organelles, in 2D dimensions: Nuclei (NU), Lysosomes (LS), Mitochondria (MT), Golgi (GL), Peroxisomes (PO), Endoplasmic Reticulum (ER), and Lipid Droplets (LDs)
The pipeline is divided in three parts:
PART-1 [CellP-2D_v1wNU_PART-1of3_03202022]:Is deputed for the identification of Nuclei, whole soma and cytoplasm.
PART-2 [CellP-2D_v1wNU_PART-2of3_03202022]: Is assigned to identify 6 organelles (LS, MT, GL, PO, ER, and LD).
PART-3 [CellP-2D_v1wNU_PART-3of3_03202022]: Measure contacts in between organelles (1 to 1), the distribution of the organelles and their contacts, and it contains the export module to csv file.
Please reffer to the notes at each module.